mRNA Library Preparation for RNA-seq

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RNA-seq allows for quantiative transcriptomic profiling of biological samples. RNA-seq takes advantage of technological advancements in high-throughput sequencing by converting RNAs of interest to a library of cDNA fragments that can then be sequenced using standard DNA-sequencing technologies. Preparing a sample for RNA-seq typically begins with either rRNA depletion of mRNA enrichment. This is due

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Genome amplification approaches: DOP-PCR, MDA, MALBAC

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DOP-PCR = degenerate oligonucleotide priming polymerase chain reaction Method Overview: In the first cycling stage (5-8 cycles), low-temperature annealing and extension occur at many binding sites in the genome and become tagged with the DOP primer. In the second cycling stage (>25 cycles), annealing temperature is raised, increasing priming specificity during amplification of the tagged

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