The Use of Orthologous Sequences to Predict the Impact of Amino Acid Substitutions on Protein Function

BY IN Comparative genomics, Functional impact prediction, Journal Club NO COMMENTS YET , ,

For nonsynonymous coding variants, functional impact prediction algorithms frequently make use of conservation of amino acid substitutions observed among homologous proteins at a given site under the assumption substitutions occurring at well-conserved sites will deleteriously impact protein function and substitutions occurring at less conserved sites will be tolerated. In this study, Marini et al. examine