mRNA Library Preparation for RNA-seq

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RNA-seq allows for quantiative transcriptomic profiling of biological samples. RNA-seq takes advantage of technological advancements in high-throughput sequencing by converting RNAs of interest to a library of cDNA fragments that can then be sequenced using standard DNA-sequencing technologies. Preparing a sample for RNA-seq typically begins with either rRNA depletion of mRNA enrichment. This is due

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RPKM and FPKM explained

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RNA-Seq provides quantitative approximations of the abundance of target transcripts in the form of counts. However, these counts must be normalized to remove technical biases inherent in the preparation steps for RNA-Seq, in particular the length of the RNA species and the sequencing depth of a sample. For example, expectedly, deeper sequencing results in higher

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