Improving RNA-Seq expression estimates by correcting for fragment bias

BY IN Journal Club, RNA-Seq NO COMMENTS YET , , , ,

Preparation steps for RNA-Seq lead to both positional bias, whereby fragments are preferential located towards either the beginning or end of transcripts, and sequence-specific bias, whereby the sequence surrounding the beginning or end of potential fragments affects their likelihood of being selected for sequencing, in sequenced fragments. If such biases are not corrected for, expression