Stupid Must Knows – Screen

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Prevent losing your work due to broken connections using screen. Especially great when working from an unstable connection. For installation and other information, see: http://www.gnu.org/software/screen/ To use screen: ssh into your remote server as usual. Actual using the command screen. You will see an introduction. Just click enter. Now you are using screen! Easy as

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Simple English Elevator Conversation Explanations: SVM

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SVM stands for support vector machine. An SVM is a supervised machine learning algorithm that’s used to analyze and recognize patterns in data for classification. This machine learning algorithm is “supervised” in the sense that we train the SVM on a set of known labeled truths, and then apply the model to predict for the

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RPKM and FPKM explained

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RNA-Seq provides quantitative approximations of the abundance of target transcripts in the form of counts. However, these counts must be normalized to remove technical biases inherent in the preparation steps for RNA-Seq, in particular the length of the RNA species and the sequencing depth of a sample. For example, expectedly, deeper sequencing results in higher

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Improving RNA-Seq expression estimates by correcting for fragment bias

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Preparation steps for RNA-Seq lead to both positional bias, whereby fragments are preferential located towards either the beginning or end of transcripts, and sequence-specific bias, whereby the sequence surrounding the beginning or end of potential fragments affects their likelihood of being selected for sequencing, in sequenced fragments. If such biases are not corrected for, expression

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